Reporter

Part:BBa_K1401616:Design

Designed by: Yash Agarwal   Group: iGEM14_BostonU   (2014-10-11)


pLac - BCD2 - tetR- GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1010
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1010
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1010
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTAGA. These were then put together using the digestion-ligation MoClo reaction. E0040m has fixed illegal cut sites


Source

E. Coli

References